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Beckman Kurt Co., Ltd. has cooperated with the national CDC to develop a rapid detection program for influenza A (H1N1)

the World Health Organization has raised the warning level of influenza A (H1N1) to level 5, which strongly indicates the upcoming pandemic. According to who, as of 11:00 on May 13, 2009, 5969 cases of influenza A (H1N1) had been confirmed worldwide and spread to 28 countries, posing a severe challenge to countries in terms of emergency early warning and epidemic control. The shear lag coefficient at the first end following the web height becomes smaller; The variation range is 2%5% of the original shear lag law After the first case of influenza A (H1N1) in Hong Kong was confirmed by road and automobile transportation, a suspected case of influenza A (H1N1) in Sichuan was confirmed on May 11. At 17:00 on May 12, Shandong reported a suspected case of influenza A (H1N1), and a suspected case of influenza A (H1N1) was found in Hong Kong on the same day. Due to the similar infection symptoms caused by the virus, it is difficult to confirm the type of infection by conventional methods. Therefore, the early and rapid diagnosis of influenza A (H1N1) is very important for the effective control of the epidemic

Beckmann Kurt has rich experience and complete solutions in the fields of virus nucleic acid extraction, laboratory automation and virus nucleic acid detection. In view of this influenza A (H1N1) epidemic, the company has cooperated with the national CDC to jointly develop a rapid diagnosis scheme for influenza A (H1N1) according to the latest H1N1 influenza virus sequence information released by the World Health Organization and the technical basis of both sides, and at the same time develop a standard scheme for detection and mutation analysis of a variety of upper respiratory viruses including human influenza, avian influenza, swine influenza, SARS, etc., which is fast, accurate, safe It has the characteristics of low cost, and can be used for rapid influenza virus screening, virus typing, mutation detection and pathological research of rapid temperature change laboratory

safe extraction of virus nucleic acid

the spri-tetm viral Na extraction kit of Beckmann Kurt company in the United States, combined with the spri-tetm automatic nucleic acid extractor, provides a fast and safe solution for the extraction of influenza A (H1N1) virus nucleic acid. Spri-tetm uses solid-phase carrier reversible magnetic beads to efficiently extract viral RNA. There is no need for manual intervention from sample cleavage to nucleic acid elution, which minimizes the risk of operators. Spri-tetm system can extract samples in a batch, and the extraction time of each batch is about 30 minutes

specific primer design

according to the influenza A (H1N1) sequence published by who, Beckman Kurt company of the United States and the national CDC jointly designed a high specific PCR amplification primer set. The primer set is designed with the express profilertm module of GeXP system, including 5 pairs of specific primers, including influenza A, H1N1, seasonal influenza, influenza A H1N1 and quality control sequence. Different from the conventional PCR primers, each pair of primers has a universal primer at the 5 'end, and the length of five groups of amplification products ranges from 100 to 600 bases

rapid detection of a/H1N1 virus

multiple amplification of 5 pairs of primers (5 pairs of influenza virus specific primers and 1 pair of internal quality control primers) using the xp-pcr technology patented by Beckman (the whole process is completed by the biomektm series of automated workstations). The multiplex PCR products can be separated and detected by capillary electrophoresis due to their different lengths. The original electrophoresis data, sample information and gene information are input into the GeXP software system for analysis, and the virus detection report of the sample can be obtained

virus typing and variation detection

the experimental data adopts the database management method. On the basis of multiple virus monitoring, standard A H1N1 virus primers are used to amplify the virus genome sequence, and the sequence analysis function of GeXP system is used to analyze the virus variation sites and possible protein sequence variations

this method can scan the possible pathogens of common influenza symptoms at one time to determine the infection type of the test sample. It has the advantages of low sample demand, high detection speed, and monitoring the variation of the virus

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